Analysis of rice cis-acting elements

In order to characterize regulatory region of oryza sativa ssp. indica and japonica in genome-scale, we mapped and collected near 600 regulatory sequences which were further used for analysis of rice cis -acting element(signal)'s amount, distribution and GC content, based on rice and arabidopsis genome and their full-length mRNA sequences under quality control. The results indicated that there were significant quantitative changes of cis -acting elements in rice regulatory region. A diverse signal usage between rice and arabidopsis were observed. Meanwhile the difference between different types of rice genes or rice specific and un-specific genes in signal usage were also recognized. Those different usages of signals resulted in fluctuation of GC content in regulatory region of genes. No significant difference in usage of cis -acting elements and GC content between two rice subspecies ( indica and japonica ) was found in this study.